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Briefly, extracted RNA was reverse transcribed to single stranded cDNA using an oligo d(T) primer linked to a T7 RNA polymerase promoter (Affymetrix). Second strand cDNA was synthesized using Escherichia coli DNA polymerase I (New England Biolabs, Ipswich, MA, USA) and RNase H (Amersham Biosciences Corp., Piscataway, NJ, USA). The first round of amplification was performed by in vitro transcription using the MegaScript Kit (Ambion Inc.).
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The rally in biotech was broad, http://www.cheap-raybans-sunglasses.combut most particularly felt in the small cap portion of the sector. Large caps actually succumbed to some profit taking on Friday while small caps continued to move forward and were up over 20% on the week. I would not be surprised if we see some consolidation this week as investors digest huge gains.